Biofabrication of Osteochondral Tissue Equivalents by Printing Topologically Defined, Cell-Laden Hydrogel Scaffolds

Biofabrication of Tissue Scaffolds

A cell-laden microfluidic hydrogel.

The encapsulation of mammalian cells within the bulk material of microfluidic channels may be beneficial for applications ranging from tissue engineering to cell-based diagnostic assays. In this work, we present a technique for fabricating microfluidic channels from cell-laden agarose hydrogels. Using standard soft lithographic techniques, molten agarose was molded against a SU-8 patterned sili...

Layer by layer three-dimensional tissue epitaxy by cell-laden hydrogel droplets.

The ability to bioengineer three-dimensional (3D) tissues is a potentially powerful approach to treat diverse diseases such as cancer, loss of tissue function, or organ failure. Traditional tissue engineering methods, however, face challenges in fabricating 3D tissue constructs that resemble the native tissue microvasculature and microarchitectures. We have developed a bioprinter that can be us...

3D printing scaffolds with hydrogel materials for biomedical applications

3D printing has now been recognized as a very practical technique to create 3D structures with milli-/micron-scale resolution. In tissue engineering, particularly, people utilize 3D printing technique to integrate biodegradable polymers to tissue scaffolds. Hydrogel is highly potential material that provides aqua environment and enables nutrition and oxygen transportation, all of which are requ...

Hydroxyapatite scaffolds for bone tissue engineering made by 3D printing.

Nowadays, there is a significant need for synthetic bone replacement materials used in bone tissue engineering (BTE). Rapid prototyping and especially 3D printing is a suitable technique to create custom implants based on medical data sets. 3D printing allows to fabricate scaffolds based on Hydroxyapatite with complex internal structures and high resolution. To determine the in vitro behaviour ...